Movie 5

Live analysis of single T cell activation
Live-cell imaging of an LGO1 cell (raw fluorescence, left; transparent coloured surface rendering, right) migrating in a 3D collagen matrix following acute addition of monomeric cognate pMHC molecules (t=0h). Surfaces coloured according to activation confidence level: grey: indeterminate, yellow: low, cyan: medium, magenta: high. Time in h:min:s. Scale bar: 10 μm.