Movie 5

Live analysis of single T cell activation
Live-cell imaging of an LGO1 cell (raw fluorescence, left; transparent coloured surface rendering, right) migrating in a 3D collagen matrix following acute addition of monomeric cognate pMHC molecules (t=0h). Surfaces coloured according to activation confidence level: grey: indeterminate, yellow: low, cyan: medium, magenta: high. Time in h:min:s. Scale bar: 10 μm.

The Lifeact-EGFP mouse is a translationally controlled fluorescent reporter of T cell activation

Jorge Luis Galeano Niño, Szun S. Tay, Jacqueline L. E. Tearle, Jianling Xie, Matt A. Govendir, Daryan Kempe, Jessica Mazalo, Alexander P. Drew, Feyza Colakoglu, Sarah K. Kummerfeld, Christopher G. Proud, and Maté Biro

J Cell Sci 2020. 133:None-None; doi: 10.1242/jcs.238014