Movie 5

Dynamics of EGFP-tagged, full length lamellipodin (EGFP-Lpd) in a migrating B16-F1 cell. B16-F1 control melanoma cell expressing EGFP-Lpd and subjected to fluorescence and phase contrast time-lapse microscopy during its migration on laminin. Note the restriction of EGFP-Lpd localization to the edges of protruding and rearward ruffling lamellipodia, but the lack of association with nascent or mature focal adhesions.

Lamellipodin tunes cell migration by stabilizing protrusions and promoting adhesion formation

Georgi Dimchev, Behnam Amiri, Ashley C. Humphries, Matthias Schaks, Vanessa Dimchev, Theresia E. B. Stradal, Jan Faix, Matthias Krause, Michael Way, Martin Falcke, and Klemens Rottner

J Cell Sci 2020. 133:None-None; doi: 10.1242/jcs.239020