Movie 1

F-actin flashes on CR-phagosomes in macrophages. LifeAct-CFP (green) was transfected into RAW 264.7 cells and cells were exposed to C3bi-sRBCs. Fluorescent and DIC images were acquired every 15 seconds with time-lapse epifluorescence imaging for 1 hour. Arrow indicates a flashing phagosome and scale bar represents 10 μm. The playback speed was 8 frames per second.