Movie 3

GFP-rGBD recruitment to LifeAct-RFP-positive CR-phagosomes in macrophages. GFP-rGBD (green) was co-transfected into RAW 264.7 cells stably expressing LifeAct-RFP (red) and exposed to C3bi-sRBCs. Fluorescent images were acquired every 45 seconds with spinning disk confocal imaging for 45 minutes. Time is shown in minutes starting from the beginning of imaging, approximately 15 minutes after particle internalization. Arrow indicates a flashing phagosome and scale bar represents 20 μm. The playback speed was 3 frames per second.