Movie 4

F-actin flashes on CR-phagosomes in macrophages exposed to CK-666. LifeAct-RFP expressing RAW 264.7 cells were exposed to C3bi-sRBCs for 15 minutes to ensure particle internalization prior to addition of 150 μM CK-666. Fluorescent images were acquired every 40 seconds with spinning disk confocal imaging for 60 minutes. Time is shown in minutes starting from the beginning of imaging, approximately 15 minutes after particle internalization. Arrow indicates a flashing phagosome and scale bar represents 20 μm. The playback speed was 3 frames per second.