Movie 2

EGFR recruitment to CCSs in the presence of Gefitinib or Batimastat. Genome-edited HeLa cells expressing endogenous mCherry-tagged μ2-adaptin were transfected with a plasmid encoding eGFP-tagged EGFR, seeded on glass, starved for 2 h and treated with 10 μM Gefitinib (left) or 10 μM Batimastat (right). Cells were then compressed under an agarose plug (1 kPa pressure) and imaged by TIRF microscopy every 15 s for 30 min. Scale bar: 10 μm.