Movie 3

TfR is lost from CCSs upon compression. Genome-edited HeLa cells expressing endogenous GFP-tagged μ2-adaptin were transfected with a plasmid encoding mCherry-tagged TfR, seeded on glass, then compressed under an agarose plug (1 kPa pressure) and imaged by TIRF microscopy every 10 s for 30 min. Scale bar: 5 μm.