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Movie 6

Targeting PTEN to the cell surface depletes PIP3 and reduces bubbling bleb formation in RD cells. Live-cell imaging of RD cells transfected with Lyn-CFP-FRB, YFP-FKBP-PTEN, and mCherry-AktPH. Rapamycin-induced YFP-FKBP-PTEN translocation inhibits bubbling blebs in RD cells.

PIP3 depletion rescues myoblast fusion defects in human rhabdomyosarcoma cells

Yen-Ling Lian, Kuan-Wei Chen, Yu-Ting Chou, Ting-Ling Ke, Bi-Chang Chen, Yu-Chun Lin, and Linyi Chen

J Cell Sci 2020. 133:None-None; doi: 10.1242/jcs.240325