iSIM imaging of actin cytoskeleton self-organization. Time-lapse series of maximum intensity projections (14 z-slices) showing actin cytoskeleton development in HFF cells expressing Lifeact-GFP plated on an 1800 μm2 adhesive fibronectin-coated island. Note the loss in fluorescence intensity by the end of imaging. Scale bar: 10 μm. Timestamp: min. Playback rate: 5 frames sec-1.