Movie 1

Characterization of putative ATP stores in cancer cells. PC3 prostate cancer cells were co-stained with a fluorescent ATP-sensing dye AS1 (white), together with a cell surface marker Alexa Fluor^®555-CTB (magenta) and actin-binding drug Alexa Fluor^®488-Phalloidin (green). Serial confocal images along the z-axis were captured using a 3i spinning disk confocal microscope CSU-W1 with Plan-Neofluar oil 63×/1.4 objective (Carl Zeiss), followed by their three-dimensional reconstruction using Imaris 8.4 software (Bitplane). For single channel view of maximum z-stack projection, see Supplementary Fig. S4.