Movie 1

Real-time multiparametric analysis of L. amazonensis-infected macrophages during pyroptosis.
Pyroptosis was induced in L. amazonensis-infected BMDMs by sequential stimulation with LPS and ATP. Real-time analyzes were performed to follow macrophage pyroptosis and amastigote localization during 240 minutes after ATP addition using the OPERA^™ plate reader at 34 °C, 7.5% CO₂. The movie was realized with sequential analyses of merged images for Ho 33342, LTG, mCherry fluorescence and TL pictures taken every 5 minutes.