Movie 2

DLD is compatible with long time-lapse imaging with minimal photobleaching and no apparent phototoxicity. CHO-K1 cells stably expressing paxillin-EGFP were seeded onto fibronectin-coated 35-mm glass bottom dishes and allowed to migrate for 16 hours in the presence of 0.0245 mW of incident light power. Images were captured with a Plan ApoChromat 20x/0.8NA objective and camera exposure time of 60,000 ms. Time is in hr:min. Scale bar is 25 μm.

Optimizing live-cell fluorescence imaging conditions to minimize phototoxicity

Alex Kiepas, Elena Voorand, Firas Mubaid, Peter M. Siegel, and Claire M. Brown

J Cell Sci 2020. 133:None-None; doi: 10.1242/jcs.242834