Movie 5

Three-dimensional imaging of mitochondrial dynamics. CHO-K1 cells stained with MitoTracker™ Red were illuminated for ~5 mins with a 561 nm diode laser set to 0.037 mW. A 15-slice z-stack with step size of 0.25 μm was captured continuously (every 1.88 s) to observe mitochondrial volume over time. Images were captured with an HCX PL APO 63x/1.40NA oil objective and camera exposure time of 50 ms. 2x2 pixel binning was used to enhance S/N. Time is in Scale bar is 5 μm.

Optimizing live-cell fluorescence imaging conditions to minimize phototoxicity

Alex Kiepas, Elena Voorand, Firas Mubaid, Peter M. Siegel, and Claire M. Brown

J Cell Sci 2020. 133:None-None; doi: 10.1242/jcs.242834