Movie 7

Continuous imaging provides superior temporal resolution for imaging actin cytoskeleton dynamics. NMuMG cells were infected with LifeAct-EGFP and seeded onto fibronectin-coated dishes. Cells were continuously illuminated for 30 mins with a 491 nm diode laser set to 0.060 mW to image EGFP fluorescence. Images were captured with an HCX PL APO 63x/1.40NA oil objective and camera exposure time of 5 s. 2x2 pixel binning was used to enhance S/N. Time series was cropped to zoom in on part of the protruding edge (white box). Time is in min:s. Scale bar on inset video series is 3 μm.