Movie 2

Region of interest during a single neutrophil transendothelial migration event, highlighting junction opening and closing. Timelapse of a single neutrophil transendothelial migration event through a TNF-α-stimulated human umbilical vein endothelial cell (HUVEC) monolayer on the single interface micropillar system (E_eff = 1.3 / 8.6 kPa), with VE-cadherin (green) and micropillars (grey). VE-cadherin was labeled by a non-function blocking antibody.