Movie 2

S2 cells expressing GalT::EGFP (green) and tdTomato::Rab6 (red) without BFA.
For two Sec71^F713Y S2 cells expressing GalT::EGFP and tdTomato::Rab6 without BFA, volumes of 49 slices of 0.25-μm thick images with 15 s interval, collected by FV3000 confocal microscopy. The area sparse with Golgi stacks were cropped and volumerendered using Fluorender to generate images from the views of three axis. Arrows indicate mergers and arrowheads indicate separations of TGNs, respectively. Scale bar: 5 μm.