Movie 3

S2 cells expressing GalT::EGFP (green) and tdTomato::Rab6 (red) after BFA treatment.
Three Sec71^F713Y S2 cells (left, middle, and right) before and after 25μM BFA addition are shown as Z-projections (upper row) and side views (lower row). Each frame represents the z-projected image of 49 slices of 0.25 μm thick images taken every 60 s for 59 min, collected by FV3000 confocal microscopy. Scale bar: 5 μm.