Movie 6

Mdm36OX cells exhibit a persistent mitochondrial tethering phenotype. Time-lapse images of Num1-GFP and Cox4-mCherry in Mdm36^OX cells showing persistent tethering of mitochondria at the brightly enhanced cortical Num1 patches. Each frame is a maximum intensity projection of 5 wide-field sections spaced 0.5 μm apart. Movie captured at 17.5 s intervals.