Movie 7

Mitochondrial network is tethered by enhanced Num1 foci in Mdm36^OX cells. Full 3D reconstruction of a deconvolved wide-field image stack showing mitochondria association with every single enhanced Num1-GFP patches in a Mdm36^OX cell. Mdm36 was overexpressed using a MET3p:MDM36 plasmid integrated at the leu2 locus. Image stack consists of 31 optical sections spaced 0.2 μm apart encompassing the entire thickness of the cell.