Movie 8

MT sliding initiates at a dim Num1^3E patch. Time-lapse images of Num1^3E-GFP and mRuby2-Tub1 in a kar9Δ cell. Frames at 0 s through 80 s show a dim cortical Num1^3E-GFP patch (arrowhead) that photobleached in later frames. Frame at 130 s shows the plus end contacting the site of the dim Num1^3E-GFP patch, followed by MT sliding in the subsequent frame. Each frame is a maximum intensity projection of 5 wide-field sections spaced 0.5 μm apart. Movie captured at 10 s intervals.