IFT trafficking in parasites isolated from the salivary glands showing first an attached epimastigote and then a metacyclic cell. Trypanosomes are expressing TdT::IFT81 following in situ tagging and display IFT trafficking. The bright signal at the base of the flagellum is clearly visible. The base and the tip of each flagellum are indicated. The video plays at real time. This video is related to Figure 5.