IFT trafficking in parasites isolated from the cardia of an infected tsetse fly. The sequence shows successively a long trypomastigote, a dividing epimastigote (trafficking visible in both old and new flagella), a long epimastigote (retrograde trains are nicely visible at the end of this sequence) and a short epimastigote with the typical accumulation of IFT proteins at the tip of the short flagellum. Cells are expressing TdT::IFT81 following in situ tagging and display IFT trafficking. The bright signal at the base of the flagellum is clearly visible. The base and the tip of each flagellum are indicated. The video plays at real time. This video is related to Figure 5.