Movie 8 (related to Fig. 3H).

Neutrophils in the optic tectum of Slc7a7-deficient larvae interact with the dying microglia. Z-scroll in dorsal view, rostral to the left, through a confocal z-stack spanning the entire OT of a cerise mutant Tg(mpeg1:mCherry; mpx:GFP) larva at 4 dpf. Neutrophils (green) appear throughout the OT, in close correlation with dying microglia (red). In the left panel, the bright-field channel is superimposed on the red and green fluorescence channels so as to show anatomical contours; the right panel shows only the overlay of red and green fuorescence channels so as to see these signals better.