Movie 1

EFA6A accumulates to the m-centriole during ciliogenesis. Arpe-19 cells transiently transfected with GFP-EFA6A, were after serum-starved for 5 or 10 min to induce ciliogenesis. Then processed for immunofluorescence using anti-gamma tubulin (magenta) and Arl13B (red) or CEP164 (yellow) antibodies. Confocal images were acquired using a Zeiss LSM780 (Zeiss GmbH), through a 63x/1.4 NA Oil objective using the Zen software (Zeiss GmbH).The images were deconvolved using Huygens Professional (version 18.10, Scientific Volume Imaging, The Netherlands,, using the CMLE algorithm, with respectively SNR:20 with 40 iterations.

EFA6A, an exchange factor for Arf6, regulates early steps in ciliogenesis

Mariagrazia Partisani, Carole L. Baron, Rania Ghossoub, Racha Fayad, Sophie Pagnotta, Sophie Abélanet, Eric Macia, Frédéric Brau, Sandra Lacas-Gervais, Alexandre Benmerah, Frédéric Luton, and Michel Franco

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.249565