Movie 2

Partial bleaching of cilia
The movie depicts a wild-type GFP-β-tubulin cell with regenerating cilia; the corresponding kymogram is shown below. For FRAP analysis, the distal ciliary segment was bleached using a focused laser beam. Note re-entry of GFP-β-tubulin into the bleached segment including numerous IFT events. The movie was recorded at 20fps and the timer show seconds.ms.