Movie 2

Primary human neutrophils were isolated from the whole blood of healthy volunteers and perfused over TNF-α-activated monolayers at a density of 1 x 10⁶ cells per mL. The video represents how efficiently neutrophils undergo full TEM in our assays (over 90% efficiency). Phase bright cells are deemed adherent, whereas phase dark cells are deemed fully transmigrated. Time code in red represents minutes, seconds and milliseconds.