To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Movie 2 Robustness of the algorithm for a free swimming spermatozoon with a very noisy background in the image and scattered debris (see t= 180 s and 260 s). Long-term segmentation-free assessment of head–flagellum movement and intracellular calcium in swimming human sperm Gabriel Corkidi, Paul Hernández-Herrera, Fernando Montoya, Hermes Gadêlha, and Alberto Darszon J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.250654 Download Original Video