Movie 4

This video illustrates how background noise and debris affected the segmentation output method (right) compared with the segmentation-free algorithm (left). The videomicroscopy data was filtered and pre-processed to allow extraction of kinematic parameters using SpermQ and OpenCASA, for comparison purposes, as displayed in Fig. 5.

Long-term segmentation-free assessment of head–flagellum movement and intracellular calcium in swimming human sperm

Gabriel Corkidi, Paul Hernández-Herrera, Fernando Montoya, Hermes Gadêlha, and Alberto Darszon

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.250654