Movie 5

Processed video-data of trypanosomes from other reports (Gad®lha et al., 2014) whose morphology and wave-kinematics are dramatically distinct from sperm swimming. Trypanosomes swim with flagellar traveling waves in the reverse direction, the flagellum “pulls” the body rather than pushing, as observed for sperm motion. This demonstrates the applicability of the method beyond human sperm motility.

Long-term segmentation-free assessment of head–flagellum movement and intracellular calcium in swimming human sperm

Gabriel Corkidi, Paul Hernández-Herrera, Fernando Montoya, Hermes Gadêlha, and Alberto Darszon

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.250654