To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Movie 6 Processed video-data of bull sperm from other reports (Kantsler et al., 2007) in microfluidic devices with distinct magnification and swimming. Again, this demonstrates the applicability of the method beyond human sperm motility. Long-term segmentation-free assessment of head–flagellum movement and intracellular calcium in swimming human sperm Gabriel Corkidi, Paul Hernández-Herrera, Fernando Montoya, Hermes Gadêlha, and Alberto Darszon J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.250654 Download Original Video