Movie 3

Corresponding to Figs. 2a and b. Cells expressing Lyn11-RFP (magenta) and GFPRab5A (cyan) imaged every 20 sec during EGF treatment. EGF addition occurred between 100-120 sec timestamps, as indicated. Arrowhead follows a macropinosome of interest. Playback rate: 4 fps. Scale bar: 5 μm. Movie is representative of ≥20 movies from 3 separate experiments.

Rab5 regulates macropinocytosis by recruiting the inositol 5-phosphatases OCRL and Inpp5b that hydrolyse PtdIns(4,5)P2

Michelle E. Maxson, Helen Sarantis, Allen Volchuk, John H. Brumell, and Sergio Grinstein

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.252411