Movie 1.

Video of maximum intensity z-stack projections from time-lapse imaging of WT and KO oocytes expressing mCherry-Trim21 (cyan) and EGFP-CDC25B (gray) while incubated with SiR-DNA (red, chromosomes) are shown. Half of the KO oocytes were injected with a rabbit polyclonal anti-GFP antibody (KO+anti-GFP) after the resumption of meiosis. MI – metaphase I, MII – metaphase II. Time points are relative to the beginning of imaging (hh:mm). The bar corresponds to 20 μm.

CDC25B is required for the metaphase I-metaphase II transition in mouse oocytes

Ivana Ferencova, Michaela Vaskovicova, David Drutovic, Lucie Knoblochova, Libor Macurek, Richard M. Schultz, and Petr Solc

J Cell Sci 2022. 135:None-None; doi: 10.1242/jcs.252924