Movie 2.
Video of maximum intensity z-projections from matured WT oocytes and oocytes treated with PD (WT+PD, KO+PD) using light-sheet microscopy. MI – metaphase I, MII – metaphase II. Oocytes expressing EGFP-CDK5RAP2 (gray, MTOCs) and H2B-mCherry (red, chromosomes) while incubated with SiR-tubulin (cyan, spindle) are shown. Time (hh:min) is relative to germinal vesicle breakdown (GVBD). The bar corresponds to 10 μm.
CDC25B is required for the metaphase I-metaphase II transition in mouse oocytes
J Cell Sci 2022. 135:None-None; doi: 10.1242/jcs.252924