Movie 4.
Video of maximum intensity z-stack projections from time-lapse light-sheet imaging of WT and KO oocytes expressing Y170A cyclin B1-VFP (gray), histone-H2B-mCherry (red, chromosomes) and stained with SiR-tubulin (cyan, microtubules) that were cultured in the presence of 0.5 μM PD0166285 (PD, WEE1/MYT1 inhibitor). Time (hh:mm) is relative to germinal vesicle breakdown (GVBD). The bar corresponds to 20 μm.
CDC25B is required for the metaphase I-metaphase II transition in mouse oocytes
J Cell Sci 2022. 135:None-None; doi: 10.1242/jcs.252924