Movie 1

LTR positive Mtb phagosomes become leaky suggesting membrane permeabilisation. Maximum projection of time-lapse confocal microscopy images showing E2Crimson-Mtb WT (red) infected iPSDM stained with Lysotracker Green (green). Frames were every 5 min, with 6 Z-slices. Time 0 represents time of phagocytosis. Images were filtered with a Gaussian blur, radius 0.8. Presented at 7 frames per second. Stills from this video are presented in figure 1.