Mtb WT induces LC3-TVS formation. iPSDM expressing GFP-LC3B (green) were infected with E2Crimson Mtb WT (red) and followed by live cell confocal microscopy with frames every 5 min. Time 0 is the frame prior to phagocytosis. Movie is a maximum projection of 7 Z slices. Scale bar 5 μm, arrow indicates bacterium of interest. Presented at 10 frames per second. Stills from this movie are presented in figure 3.
Elliott M. Bernard, Antony Fearns, Claudio Bussi, Pierre Santucci, Christopher J. Peddie, Rachel J. Lai, Lucy M. Collinson, and Maximiliano G. Gutierrez
J Cell Sci
2021. 134:None-None; doi: 10.1242/jcs.252973