Movie 5

FIB SEM reconstruction of Mtb WT partially enwrapped in an LC3 positive membrane. 3D reconstruction of FIB SEM images obtained for cell shown in figure 2. The limiting membrane of the bacteria (cyan) and LC3 positive membranes surrounding the bacteria (green) were manually segmented. Where the presence of a membrane was ambiguous a gap was left. Presented at 10 frames per second. Stills from this video are presented in figure 4.

M. tuberculosis infection of human iPSC-derived macrophages reveals complex membrane dynamics during xenophagy evasion

Elliott M. Bernard, Antony Fearns, Claudio Bussi, Pierre Santucci, Christopher J. Peddie, Rachel J. Lai, Lucy M. Collinson, and Maximiliano G. Gutierrez

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.252973