Movie 8

FIB SEM reconstruction of Mtb WT enwrapped in an LC3 positive membrane. 3D reconstruction of FIB SEM images obtained for ROI3 in figure 4. The limiting membrane of the bacteria (red), LC3 positive membranes (green), ER-like sheet of membrane (cyan and blue) and an intraluminal vesicle (magenta) were manually segmented. Where the presence of a membrane was ambiguous a gap was left. Presented at 15 frames per second. Stills from this video are presented in figure 5