Movie 2

Repetitive [Ca²⁺]_fla elevations influence pausing of retrograde IFT.
TIRF imaging of [Ca²⁺]_fla (G-GECO) in a flagellum on a 0.1% poly-lysine treated surface. In the initial period no [Ca²⁺]_fla elevations are observed. After 40 s a series of repetitive [Ca²⁺]_fla elevations are observed.