Movie 3

Repetitive [Ca2+]fla elevations influence pausing of retrograde IFT.
TIRF imaging of IFT54-mScarlet in the flagellum shown in Video 2. In the initial period where no [Ca2+]fla elevations were observed (Movie 2), IFT particles pause and accumulate in distal regions and very few retrograde IFT trains can be observed. After 40 s, when the series of repetitive [Ca2+]fla elevations can be observed in Movie 2, the movement of IFT trains is noticeably different, with distinct retrograde IFT trains present.

Ca2+ elevations disrupt interactions between intraflagellar transport and the flagella membrane in Chlamydomonas

Cecile Fort, Peter Collingridge, Colin Brownlee, and Glen Wheeler

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.253492