Movie 4
[Ca2+]fla elevations prevent IFT-driven microsphere movement.
TIRF imaging of [Ca2+]fla (G-GECO) in a flagellum on a 0.1% poly-lysine treated surface. A large [Ca2+]fla elevation can be observed at 2.0 s.
Ca2+ elevations disrupt interactions between intraflagellar transport and the flagella membrane in Chlamydomonas
J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.253492