To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Movie 4 [Ca2+]fla elevations prevent IFT-driven microsphere movement.TIRF imaging of [Ca2+]fla (G-GECO) in a flagellum on a 0.1% poly-lysine treated surface. A large [Ca2+]fla elevation can be observed at 2.0 s. Ca2+ elevations disrupt interactions between intraflagellar transport and the flagella membrane in Chlamydomonas Cecile Fort, Peter Collingridge, Colin Brownlee, and Glen Wheeler J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.253492 Download Original Video