To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Movie 4 HeLa cells transfected with mCh-Rab5 and Rab7T22N. This movie demonstrates the detachment and reattachment of mCh-Rab5. mCh-Rab5 positive endosomes are unable to fully detach due to the lack of Rab7 recruited to the maturing endosome. Fig. 2B movie. De novo formation of early endosomes during Rab5-to-Rab7a transition Frode Miltzow Skjeldal, Linda Hofstad Haugen, Duarte Mateus, Dominik M. Frei, Anna Vik Rødseth, Xian Hu, and Oddmund Bakke J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.254185 Download Original Video