To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Movie 5 HeLa cells transfected with EGFP-Rab5 and mApple-Rab7a. The movie demonstrates the gradual exchange between EGFP-Rab5 and mApple-Rab7a and shows specifically the convergence of microdomains that ends up with the formation of a novel EGFP-Rab5 positive endosome. Fig. 3B movie De novo formation of early endosomes during Rab5-to-Rab7a transition Frode Miltzow Skjeldal, Linda Hofstad Haugen, Duarte Mateus, Dominik M. Frei, Anna Vik Rødseth, Xian Hu, and Oddmund Bakke J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.254185 Download Original Video