Movie 14

Example of an ESC dividing at the periphery of a 2D colony plated on laminin. Time-lapse (spinning-disk confocal microscopy) images of a colony of ESCs expressing H2B-RFP (red) and labeled with CellMask^TM deep red (cyan), plated on laminin (see Materials and Methods section) with one cell dividing at the periphery of the colony. One frame is shown every 5 min. One Z plane is shown. Scale bar: 10 μm.