Movie 14

Example of an ESC dividing at the periphery of a 2D colony plated on laminin. Time-lapse (spinning-disk confocal microscopy) images of a colony of ESCs expressing H2B-RFP (red) and labeled with CellMaskTM deep red (cyan), plated on laminin (see Materials and Methods section) with one cell dividing at the periphery of the colony. One frame is shown every 5 min. One Z plane is shown. Scale bar: 10 μm.

Three-dimensional geometry controls division symmetry in stem cell colonies

Agathe Chaigne, Matthew B. Smith, Rocio Lopez Cavestany, Edouard Hannezo, Kevin J. Chalut, and Ewa K. Paluch

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.255018