Movie 1

Electron tomography and 3D reconstruction of a thick section from a chemically-fixed VAMP7+VAMP8 double knockout HeLa cell revealing the accumulation of small vesicles (pink) surrounding two MVB's (cyan). Some of the vesicles have clearly-defined tethers (green). The flat clathrin patches (blue) are devoid of attached vesicles. Tubular elements apposed to, but not contiguous with, MVBs are likely to be smooth ER (white). Scale bar 200nm.