Movie 1.

3D reconstruction of apical surfaces of MDCK cysts. Six-day old MDCK cysts were induced with doxycycline at day 3 were fixed and stained with ZO-1 (red) and gp135 (white) and whole cysts were imaged with confocal microscope. 3D projection was constructed from all the confocal slices and depicted as 360° view. GFP fluorescence associated with EREG is excluded from the reconstructed images to highlight the underlying apical surfaces.