Movie 1.
3D reconstruction of apical surfaces of MDCK cysts. Six-day old MDCK cysts were induced with doxycycline at day 3 were fixed and stained with ZO-1 (red) and gp135 (white) and whole cysts were imaged with confocal microscope. 3D projection was constructed from all the confocal slices and depicted as 360° view. GFP fluorescence associated with EREG is excluded from the reconstructed images to highlight the underlying apical surfaces.
Induction of apically mistrafficked epiregulin disrupts epithelial polarity via aberrant EGFR signaling
J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.255927