Movie 1

Supports Figures 3A and 3B: depicts the large 3D data sets acquired and the segmentation and analysis method employed to calculate Mitophagy Index using a representative 7 dpf fasted ubi:mitoKeima larva. Keima440 is in green, Keima561 is in magenta. At the beginning of the movie, a 3D rendered maximum intensity projection is displayed and rotated. Then, the ratiometric image derived from Keima561/Keima440 is shown. Lastly, the isosurface rendering objects are shown. The magenta surface objects represent mitolysosomes and the green surface object represents the total tissue volume analyzed.