Movie 2.
Three-dimensional (3D) reconstruction of MII spindles in control WT and Tg oocytes. Laser scanning confocal 3D reconstruction of Z-stack images captured at 0.5μm intervals using a 40x oil objective and 6x digital zoom. Ovulated MII oocytes collected from WT and Tg mice were fixed after a 4h culture in media alone, then immunolabeled to detect TACC3 (green) and PCNT (red) at aMTOCs, and the chromosomes (blue, DAPI).
Loss of acentriolar MTOCs disrupts spindle pole Aurora A and assembly of the liquid-like meiotic spindle domain in oocytes
J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.256297