Movie 3.

SR-SIM sections of MII spindle poles in control WT and Tg (PCNT-depleted) oocytes. Z-stacks of structurally illuminated super-resolved images (91 um intervals) acquired on the ELYRA S1 SR-SIM microscope using NIS Elements software. Ovulated MII oocytes collected from WT and Tg (PCNT-depleted) mice were fixed after a 4h culture in media alone, then immunolabeled to detect TACC3 (green), and PCNT (red).