Movie 4.

Confocal sections of MII spindles in WT and Tg (PCNT-depleted) oocytes following AURKA inhibition. Z-stacks were imaged at 0.5μm intervals with a 40x oil objective and 6x digital zoom. Ovulated MII oocytes from WT and Tg mice were fixed after a 4h culture in media supplemented with MLN8237 (500nM) to inhibit AURKA, then immunolabeled to detect TACC3 (green), PCNT (red) at aMTOCs, and the chromosomes (blue, DAPI).